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Proliferation and differentation of normal granulopoietic cells in continuous bone marrow cultures
Author(s) -
Williams Neil,
Jackson Heather,
Rabellino Enrique M.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040930315
Subject(s) - bone marrow , stem cell , granulocyte , biology , receptor , immunology , macrophage , chemistry , cancer research , microbiology and biotechnology , in vitro , biochemistry
Modified conditions are reported for successful continuous bone marrow cultures with stem cell self‐renewal and granulocyte‐macrophage differentiation. Cells cultured over several weeks were found to be identical to freshly isolated bone marrow cells. Polymorphic neutrophils derived from cultures and primary bone marrow neutrophils both showed C 3 and IgG receptors and both actively phagocytosed foreign particles. Cultured and normal CFU‐c were identical, both in their dose responsiveness to CFS and in their sedimentation rate characteristics.