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Dibutyryl cyclic AMP arrests the growth of cultivated cloudman melanoma cells in the late S and G2 phases of the cell cycle
Author(s) -
Dipasquale Albert,
McGuire Joseph
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040930311
Subject(s) - dna synthesis , cell division , feulgen stain , cell cycle , cell , cell growth , biology , dna , microbiology and biotechnology , thymidine , biochemistry
DBcAMP reversibly arrests cultivated Cloudman melanoma cells in the late S and G2 phases of the cell cycle. This is supported by the measurement of DNA synthesis by autoradiography and measurement of cellular DNA by two methods—the diphenylamine reaction and microspectrophotometry of Feulgen stained cells. We also present evidence that (1) cell division is prevented if DBcAMP is added as late in the cycle as early S phase. (2) The inhibition of cell division does not appear to be caused by products of tyrosine oxidation. (3) The increase in cell size that occurs in the presence of DBcAMP reflects continued synthesis of protein in the absence of cell division.