Separation of proximal tubule cells from suspensions of rat kidney cells by free‐flow electrophoresis | Zendy

Kreisberg Jeffrey I. | Zendy; Sachs George | Zendy; Pretlow Thomas G. | Zendy; McGuire Robert A. | Zendy

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Separation of proximal tubule cells from suspensions of rat kidney cells by free‐flow electrophoresis

Author(s) -

Kreisberg Jeffrey I.,

Sachs George,

Pretlow Thomas G.,

McGuire Robert A.

Publication year - 1977

Publication title -

journal of cellular physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.529

H-Index - 174

eISSN - 1097-4652

pISSN - 0021-9541

DOI - 10.1002/jcp.1040930121

Subject(s) - microbiology and biotechnology , electrophoresis , brush border , free flow electrophoresis , trypsin , chemistry , kidney , tubule , flow cytometry , biology , chromatography , polyacrylamide gel electrophoresis , biochemistry , gel electrophoresis of proteins , endocrinology , membrane , enzyme , vesicle

Suspensions of rat kidney cells obtained by disaggregation of the kidney with 0.25% trypsin were separated by electrophoresis. Previously, we found a correlation between cells with histochemically demonstrable alkaline phosphatase (HDAP) and cells with brush borders which established that HDAP is a useful marker for rat proximal tubule cells (Kreisberg et al., '77). The starting suspension of cells for electrophoresis consisted of 38.4 ± 5.7% nucleated cells with HDAP, 39.8 ± 5.7% nucleated cells without HDAP, and 20.8 ± 9.2% red blood cells. After electrophoresis, the purest fraction contained 85.8 ± 3.5% nucleated cells with HDAP, 8.4 ± 2.2% nucleated cells lacking HDAP, and 5.8 ± 2.8% red blood cells; 91.9 ± 2.4% of the nucleated cells in the purest fractions had HDAP.

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