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Resistance of teratocarcinoma stem cells to infection with simian virus 40: Early events
Author(s) -
Swartzendruber Douglas E.,
Friedrich Thomas D.,
Lehman John M.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040930105
Subject(s) - teratocarcinoma , biology , virus , stem cell , virology , cell culture , somatic cell , antigen , microbiology and biotechnology , cellular differentiation , immunology , genetics , gene
Multipotential stem cells of a murine teratocarcinoma are resistant to typical infection with either polyoma virus (PV) or Simian virus 40 (SV 40 ). Differentiated progeny of the stem cells are susceptible to infection in a manner identical to other mouse somatic cells, i.e., they are permissive for PV and nonpermissive for SV 40 . The early interactions between the stem cells (embryonal carcinoma or EC cells) and SV 40 and PV were studied. Virions adsorbed to and penetrated into the cytoplasm and nucleus of EC cells, but did not induce expression of T antigen in the EC nuclei. Purified SV 40 DNA was capable of inducing T antigen in differentiated teratocarcinoma cells but not in EC cells. Virus could not be rescued from EC cells previously exposed to SV 40 . The resistance of the stem cells to infection apparently involves a block in the infectious cycle after adsorption and penetration but before T antigen induction.