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Membrane transport by macrophages in suspension and adherent to glass
Author(s) -
Pofit Joseph F.,
Strauss Phyllis R.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040920213
Subject(s) - suspension (topology) , cytochalasin b , colchicine , membrane , biophysics , chemistry , adenosine , suspension culture , cytochalasin , microbiology and biotechnology , biochemistry , biology , cell culture , cell , cytoskeleton , genetics , mathematics , homotopy , pure mathematics
Abstract We have compared membrane transport of lysine and adenosine by rabbit lung macrophages in suspension and adherent to glass cover slips. The rapid sampling techniques employed permitted measurements to be made over intervals as short as 10 seconds. Suspended cells transported both nutrients at a faster rate, although the Km values for the two transport systems remained unchanged. Colchicine, cytochalasin B and adenosine did not interfere with the enhancement.