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Rapid cell surface‐related stimulation of alkaline phosphatase in HeLa cells by dimethyl DL‐2,3‐distearoyloxypropyl‐2′‐hydroxyethylammonium acetate (Rosenthal's inhibitor)
Author(s) -
Melnykovych G.,
Lopez I. C.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040920111
Subject(s) - alkaline phosphatase , hela , strain (injury) , stimulation , enzyme , biochemistry , phosphatase , chemistry , acid phosphatase , cell , cell culture , microbiology and biotechnology , biology , endocrinology , anatomy , genetics
Cultures of HeLa cells, strains S3G (HeLa 65 ) and S3K (HeLa 71 ) were grown in plastic dishes until firmly attached and were then treated with sonic dispersions of Rosenthal's phospholipase inhibitor. A rapid increase in alkaline phosphatase activity occurred following this treatment in the S3G strain (low inducible alkaline phosphatase) but not in the S3K strain (high constitutive alkaline phosphatase). The stimulatory effect was dependent on the presence of bovine serum in the medium. No stimulation of alkaline phosphatase was observed in a variety of soluble preparations of this enzyme.