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Development of spike potentials in skeletal muscle cells differentiated in vitro from chick embryo
Author(s) -
Kano Masaakira,
Yamamoto Mitsue
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040900307
Subject(s) - myogenesis , depolarization , membrane potential , tetrodotoxin , spike potential , myocyte , biophysics , biology , sodium channel , microbiology and biotechnology , resting potential , spike (software development) , electrophysiology , in vitro , chemistry , sodium , neuroscience , biochemistry , management , organic chemistry , economics
Abstract The development of spike potential mechanisms during cell differentiation was studied in chick myotubes formed in vitro from trypsin‐dissociated myoblasts. The spike potential and its rate of rise were measured in myotubes from 4–14 day old cultures. A depolarizing current pulse was delivered to evoke the spike potential after the steady membrane potential had been adjusted to a standard level of −80 mV in all cases. This gives the greatest maximum rate of rise of the spike potential and eliminates variation due to differences in the resting membrane potential of the myotubes. The size and maximum rate of rise of the spike potential increased significantly during the period examined. The spike potential was blocked by tetrodotoxin in almost all myotubes. These results suggest that during differentiation myotubes develop the ability to generate a spike potential due to an inward current carried by sodium ions.