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Growth and biochemical characteristics of a detachment variant of CHO cells
Author(s) -
Atherly Alan G.,
Barnhart Benjamin J.,
Kraemer Paul M.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040900302
Subject(s) - biology , chinese hamster ovary cell , microbiology and biotechnology , chemistry , computational biology , genetics , cell culture
A variant subline of Chinese hamster cells (line CHO) was isolated that had increased resistance to detachment from the substratum. Comparisons between parental and variant cells of the complex carbohydrates liberated during trypsin detachment showed that the variant cells synthesized little or no hyaluronic acid. These cells also had reduced amounts of other complex carbohydrates in the cell periphery. However, parental and variant cells did not differ in morphology, growth control, or cyclic AMP concentration. Profound changes in the physical and chemical nature of the cell periphery, in themselves, evidently are insufficient to cause changes in many aspects of cell behavior.