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Studies on intercellular adhesion. I. Adhesion of neural retina cells to isotypic aggregates in serum free medium
Author(s) -
Pessac Bernard,
Alliot Françoise,
Girard Arlette
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040900104
Subject(s) - adhesion , intracellular , cell adhesion , microbiology and biotechnology , chemistry , divalent , biophysics , cell , neural cell adhesion molecule , retina , sodium , biology , biochemistry , neuroscience , organic chemistry
A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., 1971) is described. The use of mechanically dissociated single cells labeled with 3 H‐leucine, and of a gyratory shaker increased considerably the efficiency of cell collection. With chick embryo neural retina, it was shown that isotypic cell adhesion occurs in simple synthetic media and even in sodium chloride solution suggesting that divalent cations do not play a major role in adhesion of these cells. Cell collection in media without serum was not affected by metabolic or protein synthesis inhibitors. However, cell adhesion was temperature‐dependent since no collection occurred at 4°C but was maximal at 38°C.