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Nature of lectin‐induced alteration of potassium transfer in Ehrlich ascites tumor cells
Author(s) -
Aull Felice,
Nachbar Martin S.,
Oppenheim Joel D.
Publication year - 1977
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040900103
Subject(s) - ricin , concanavalin a , lectin , efflux , potassium , chemistry , biochemistry , ouabain , atpase , ehrlich ascites , microbiology and biotechnology , biology , tumor cells , sodium , enzyme , toxin , in vitro , organic chemistry , cancer research
The way in which the lectins concanavalin A (Con A) and Ricinus communis agglutinin (Ricin) alter the K + content of Ehrlich ascites tumor cells was investigated. Unidirectional and net fluxes were determined in unwashed cells during a time course following lectin addition. Total influx, ouabain sensitive influx, Mg ++ ‐ and Na + ‐K + ‐ATPase activity were all unaffected. Cell ATP content was normal for at least 19 minutes after exposure to Con A. Early after contact with Ricin or Con A efflux was stimulated 2‐3‐fold, resulting in net K + loss, but after 20 minutes efflux had returned to normal. Ricin and Con A acted similarly although Ricin was present at only 1/50 the concentration of Con A. When the findings are evaluated together with previous work it is suggested that a particular membrane glycoprotein may be concerned in the efflux alteration observed.