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Cooperative binding of concanavalin a to thymocyte membrane vesicles augments uptake of α‐aminoisobutyrate
Author(s) -
SchmidtUllrich Rupert,
Mann Nathan D.,
Wallach Donald F. H.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890442
Subject(s) - concanavalin a , lectin , vesicle , membrane , thymocyte , biology , chemistry , biophysics , biochemistry , immunology , in vitro , antigen , cd8
(1) Membrane vesicles from rabbit thymocytes accumulate α‐aminoisobutyrate in the presence of 0.1 M NaCl. Uptake is 1/2 maximal after about 2 min and reaches a plateau value (61 pmoles/mg protein) after 30 min. (2) Up to 25 μg concanavalin A/ml, binding of the lectin describes a sigmoid curve indicative of a cooperative process. (3) At lectin concentrations up to 8 μg/ml, lectin binding enhances the uptake of α‐aminoisobutyrate (maximally 30%).