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Neoplastic potentials and regulation of uptake of nutrients. I. A glutamine independent variant of polyoma bhk with a very high neoplastic potential
Author(s) -
Gammon Maureen T.,
Isselbacher Kurt J.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890438
Subject(s) - glutamine , concanavalin a , biology , baby hamster kidney cell , cell culture , cell , biochemistry , microbiology and biotechnology , amino acid , genetics , in vitro
A variant of polyoma BHK cells is described which is capable of growing in a glutamine‐free medium. These glutamine‐independent variant cells (GIV) have a size and morphologic appearance intermediate between the nontransformed (BHK) and polyoma transformed (Py6) cell line. In contrast to Py6, the GIV cells grow more slowly, produce less lactic acid and do not grow in suspension. However, their adherence to substrate and release of galactosyl‐transferase into the medium is comparable to that seen with the parent PyBHK. Most striking is the finding that GIV cells are more readily agglutinated by Concanavalin A and when inoculated into hamsters are much more tumorigenic than the parent Py6 cells.