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Glucose transport and metabolism in cultured cells of nervous tissue
Author(s) -
Passonneau Janet V.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890428
Subject(s) - metabolism , nervous tissue , nervous system , carbohydrate metabolism , tissue culture , biochemistry , microbiology and biotechnology , biology , chemistry , neuroscience , in vitro
The effect of the concentration of glucose in the medium on the intracellular concentrations of metabolites in C‐6 astrocytoma cells and C‐1300 neuroblastoma cells in culture has been investigated. The intracellular concentrations of glucose, glycogen, glucose 6‐P and UDP‐glucose were measured at intervals after feeding the cells. A rapid increase in glucose and glucose 6‐P levels occurred when fresh medium containing 5.5 mM glucose was applied to the cells, followed by slower increases in UDP‐glucose and glycogen. When the medium glucose was increased ten‐fold, the intracellular concentration of glucose was increased, but the levels of glucose 6‐P, UDP‐glucose and glycogen were not altered, nor were the rates of accumulation. The addition of insulin to the medium resulted in an increase of intracellular glucose, glucose 6‐P and glycogen. The transport of glucose into the cells is not the rate‐limiting step of the regulation of metabolite levels in the cells.