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Regulation of glucose transport in chick fibroblasts: Bicarbonate, lactate and ascorbic acid
Author(s) -
Amos H.,
Christopher C. W.,
Musliner T. A.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890425
Subject(s) - ascorbic acid , bicarbonate , biochemistry , chemistry , glucose transporter , food science , biology , endocrinology , insulin , organic chemistry
The rate at which chick embryo fibroblasts in primary or secondary culture transport glucose or 3‐O‐methyl glucose is strongly influenced by the presence of bicarbonate ion in the culture medium. Cells growing or maintained on glucose at physiologic concentration (5.5mM) have an 8 to 10‐fold higher rate of glucose uptake than their counterparts cultivated without bicarbonate. These cells also produce more lactate as a consequence of their more rapid intake of glucose. The hydrogen acceptors, methylene blue and dehydroascorbate added to the culture medium reduce the cell capacity to transport glucose and 3‐O‐methyl glucose to levels obtaining in the bicarbonate‐free medium. There is a concomitant reduction in glucose utilized by cells during 24 hours and a further reduction in lactate formed per molecule of glucose metabolized.