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Magnesium deprivation reproduces the coordinate effects of serum removal or cortisol addition on transport and metabolism in chick embryo fibroblasts
Author(s) -
Rubin H.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890418
Subject(s) - embryo , metabolism , endocrinology , magnesium , medicine , hydrocortisone , fibroblast , biology , chemistry , microbiology and biotechnology , andrology , biochemistry , in vitro , organic chemistry
A variety of unrelated effectors stimulate or inhibit coordinately the same array of metabolic reactions in chick embryo fibroblasts, including the uptake of 2‐deoxy‐D‐glucose and uridine, and the incorporation of uridine and thymidine into acid insoluble material. The coordinate inhibition of these reactions by omission of serum or addition of cortisol is reproduced quantitatively by lowering the concentration of magnesium (Mg 2+ ) in medium containing 0.2 mM Ca 2+ . The response times for the utilization of uridine and thymidine following the removal or addition of Mg 2+ are similar to those which follow removal or addition of serum. The effect of serum on the incorporation of choline, which is not part of the coordinate response to unrelated effectors, is not reproduced by varying Mg 2+ concentrations. The results support the hypothesis that the availability of Mg 2+ within the cell plays a central role in the coordinate control of transport, metabolism and growth by external physiological effectors.