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Cell envelope proteins involved in the transport of maltose and sn ‐glycerol‐3‐phosphate in Escherichia coli
Author(s) -
Boos Winfried
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890407
Subject(s) - escherichia coli , cell envelope , maltose , glycerol , chemistry , phosphate , biochemistry , escherichia coli proteins , envelope (radar) , maltose binding protein , cell , sucrose , recombinant dna , fusion protein , gene , telecommunications , radar , computer science
Two types of proteins are discussed in their role of facilitating the transport of maltose and sn ‐glycerol‐3‐phosphate in E. coli . The first protein is the receptor for phage δ, known to be an outer membrane protein. By facilitating the diffusion of maltose and the higher maltodextrins through the outer membrane the effect of the δ receptor is to decrease the K m of the transport system without influencing the V max of substrate flux. The second protein is a periplasmic protein that is induced by growth on glycerol and is essential for transport of sn ‐glycerol‐3‐phosphate in whole cells but not in membrane vesicles. This protein has solely been identified by the use of a two‐dimensional polyacrylamide gel electrophoresis of periplasmic proteins in wild‐type and mutants defective in sn ‐glycerol‐3‐phosphate transport.