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Density regulation of amino acid transport in cultured, androgen‐responsive tumour cells
Author(s) -
Robinson Jeffery H.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040890110
Subject(s) - sodium , amino acid , chemistry , biochemistry , biophysics , membrane transport , membrane , biology , organic chemistry
In androgen‐responsive cells, activity of a sodium‐dependent transport system for neutral amino acids is shown to be density‐dependent, whereas activity of a sodium‐independent system is not. Transport of the nonmetabolizable amino acid, α‐aminoisobutyric acid (α‐aib) was largely sodium‐dependent, whereas transport of cycloleucine (cyleu) was by both sodium‐dependent and sodium‐independent systems. The K m for cyleu transport changed with density because only the sodium‐dependent portion of this transport was density‐dependent. By contrast, the V max for α‐aib transport changed with density, indicating that either the amount of functional membrane carrier or the activity of the sodium pump was affected.