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Cellular responsiveness to stimulation in vitro: Increased responsiveness to colony stimulating factor of bone marrow colony‐forming cells treated with surface‐active agents and cyclic 3′5′ AMP
Author(s) -
Fleming W. A.,
McNeill T. A.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880308
Subject(s) - colony stimulating factor , stimulation , colony forming unit , bone marrow , in vitro , biology , agar , macrophage colony stimulating factor , endocrinology , granulocyte , growth factor , immunology , haematopoiesis , andrology , medicine , microbiology and biotechnology , macrophage , biochemistry , stem cell , receptor , bacteria , genetics
Addition of low concentrations (10 ng/ml) of saponin or Tween 80 to stimulated cultures of normal mouse bone marrow in agar increased the number of granulocyte‐macrophage colonies which developed. Addition of cyclic AMP or dibutyryl cyclic AMP in low concentration (10 −8 to 10 −10 M) also enhanced colony numbers although concentrations above 10 −5 M were inhibitory. Enhancement was found when marrow cells were pre‐treated with these agents and cultured in their absence. The agents did not stimulate colony development in the absence of colony‐stimulating factor and enhancement of colony number occurred only in cultures containing a concentration of colony‐stimulating factor which was sub‐optimal in terms of maximum colony development. There was no indication of increased colony‐stimulating factor production by treated marrow cells under the experimental conditions used to show colony enhancement. It was concluded that the agents caused an increased responsiveness of colony‐forming cells to colony‐stimulating factor.