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Increased susceptibility of murine teratocarcinoma cells to simian virus 40 and polyoma virus following treatment with 5‐bromodeoxyuridine
Author(s) -
Speers Wendell C.,
Lehman John M.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880305
Subject(s) - teratocarcinoma , bromodeoxyuridine , biology , virus , embryonal carcinoma , microbiology and biotechnology , cell culture , virology , embryo , cellular differentiation , immunology , immunohistochemistry , biochemistry , genetics , gene
Abstract Cultures of the multipotential stem cell, embryonal carcinoma (EC), of a murine teratocarcinoma were treated with 5‐bromodeoxyuridine (BrdU). Within 2–4 days at concentrations of 1–50 m̈gm/ml of BrdU, there was a marked change in the morphology of cells observed by light and electron microscopy. A comparison of the growth potential showed that for up to four days the BrdU‐treated cultures were similar to untreated cultures. When these BrdU‐treated cells were infected with Simian virus 40 (SV 40 ) and polyoma virus (Py), there was an increase in susceptibility of the treated cells. The untreated embryonal carcinoma cells were refractory. These results suggest that BrdU modifies the embryonal carcinoma cells to allow infection with two DNA viruses.