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Establishment in culture of a multihormone‐secreting cell strain derived from the mtT/F 4 rat pituitary tumor
Author(s) -
Richardson U. Ingrid
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880304
Subject(s) - prolactin , endocrinology , biology , medicine , pituitary tumors , hormone , cell culture , prolactin cell , mtt assay , population , cell growth , secretion , doubling time , receptor , anterior pituitary , cell , biochemistry , genetics , environmental health
A clonal cell strain F 4 C 1 has been established from the transplantable rat pituitary tumor MtT/F 4 and has been maintained in continuous culture for two years. The cells grow with a population doubling time of 48 hours; the karyotype with a modal number of 39 chromosomes includes a pair of large metacentric marker chromosomes. F 4 C 1 cells in culture produce growth hormone and prolactin but not adrenocorticotropin in contrast to the MtT/F 4 tumor which secretes all three hormones in the host rat. The cloned cells lack specific receptors for thyrotropin‐releasing hormone and do not respond to this agent with increased prolactin or decreased growth hormone production. Treatment with hydrocortisone results in a small increase in growth hormone and a small decrease in prolactin production. Tumors generated in rats from injected F 4 C 1 cells secrete prolactin and growth hormone but not adrenocorticotropin. The results suggest that growth hormone and prolactin are produced by a single cell type in the MtT/F 4 tumor.