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Synthesis of immunoglobulin by substrate attached mouse myeloma cells
Author(s) -
Bargellesi Antonio,
Damiani Guido,
Kuehl W. Michael,
Scharff Matthew D.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880214
Subject(s) - antibody , substrate (aquarium) , multiple myeloma , chemistry , biology , microbiology and biotechnology , immunology , ecology
Cultured mouse myeloma cells grow in suspension and synthesize and secrete large amounts of immunoglobulin. Mouse myeloma cells which attach to a plastic substratum have been obtained by mutagenesis and subsequent selection. Normal mouse myeloma cells will also attach to plastic tissue culture dishes pre‐treated with poly‐L‐lysine. The attached cells synthesize and secrete the same large amounts of immunoglobulin as the suspended cells.