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Wasting of 18 S ribosomal RNA by human myeloma cells cultured in adenosine
Author(s) -
Bynum John W.,
Volkin Elliot
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880209
Subject(s) - wasting , adenosine , ribosomal rna , multiple myeloma , ribosome , rna , microbiology and biotechnology , chemistry , biology , cancer research , biochemistry , gene , immunology
Abstract When human myeloma cells are pulsed for one hour with 3 H‐uridine and chased for six hours in fresh medium containing unlabeled uridine, the processing of 45 S rRNA precursor into the stable 28 S and 18 S rRNA components can be followed. However, when the cells are chased in exogenous adenosine instead of uridine, the accumulation of 18 S rRNA is selectively inhibited. Cells pulsed with 3 H‐adenosine and chased in the absence of exogenous nucleosides exhibit normal rRNA precursor processing, while cells pulsed simultaneously with 3 H‐uridine and 3 H‐adenosine and chased with uridine and adenosine are deficient in labeled 18 S rRNA. Consequently, the inhibition of 18 S rRNA accumulation by adenosine is not an artifact of labeling nor is it relieved by an equal molar concentration of uridine. The wasting of 18 S rRNA in human myeloma cells is similar to that reported to occur in normal lymphocytes during the quiescent state.