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SV40 transformation of mouse brain cells: Critical role of gene A in maintenance of the transformed phenotype
Author(s) -
Anderson Jeffrey L.,
Martin Robert G.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880109
Subject(s) - phenotype , cell culture , biology , transformation (genetics) , sv40 large t antigen , mutant , microbiology and biotechnology , brain cell , gene , doubling time , cell growth , transfection , genetics
Brain cells derived from the NIH Swiss mouse strain have been established in tissue culture. Astrocytic neuroglial cells, identified by morphology and staining properties, predominate. The brain cell culture was successfully transformed with SV40 wild type virus and with a representative early ( A239 ) and late ( C219 ) mutant. When subjected to growth analysis the A239 transformant displayed selective loss of six characteristics of the transformed phenotype at the restrictive temperature (40.5°C): doubling time, saturation density, ability to grow in low serum, efficiency of growth on plastic and on normal brain cell layers, and cloning in soft agar. Temperature shift experiments demonstrated the reversibility of the differences in saturation density. T‐antigen was expressed at both temperatures. Alteration in uptake of 2‐deoxyglucose was not a characteristic of the transformed phenotype. The cell lines may be of utility in brain culture work and in studies on the mechanism of SV40 transformation.