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Human lymphocyte potassium content during the initiation of phytohemagglutinin‐induced mitogenesis
Author(s) -
Segel George B.,
Lichtman Marshall A.,
Hollander Marsha M.,
Gordon Bruce R.,
Klemperer Martin R.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040880106
Subject(s) - extracellular , lymphocyte , potassium , biology , medicine , chemistry , immunology , endocrinology , microbiology and biotechnology , biochemistry , organic chemistry
The K + content of human lymphocytes has been examined during the initial 24 hours after exposure of cells to phytohemagglutinin (PHA). We have reconfirmed that lymphocyte K + exchanges rapidly for extracellular counterions during preparative washing if cells are exposed to PHA. By using a technique to measure cation content which does not require removal of cells from their culture medium, we have shown that K + does not change for 24 hours following PHA treatment. Previous reports have demonstrated that an enhanced uptake of K + occurs in lymphocytes treated with PHA. This increased uptake may be a compensatory change for an increased exodus, explaining the failure of K + to change following lectin treatment.