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Exodus of 42 K + and 86 Rb + from rat thymic and human blood lymphocytes exposed to phytohemagglutinin
Author(s) -
Segel George B.,
Gordon Bruce R.,
Lichtman Marshall A.,
Hollander Marsha M.,
Klemperer Martin R.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870309
Subject(s) - incubation , efflux , intracellular , lymphocyte , microbiology and biotechnology , potassium , isotopes of potassium , chemistry , biology , radiochemistry , biochemistry , immunology , organic chemistry
We have found that PHA produces an alteration in the lymphocyte membrane which allows 86 Rb + or 42 K + in prelabeled lymphocytes to exchange for cations present in washing solutions. These observations suggested that PHA might induce an increase in the exodus of intracellular potassium during incubation in physiologic media. We, therefore, examined 86 Rb + and 42 K + efflux from rat and human lymphocytes during incubation in tissue culture medium. The rate constant for efflux, K e , was significantly increased by PHA. 86 Rb + efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment.