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Effect of exogenous atp on sodium transport in mammalian red cells
Author(s) -
Romualdez A.,
Volpi M.,
Sha'afi R. I.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870305
Subject(s) - gtp' , adenosine triphosphate , adenosine , nucleotide , biophysics , chemistry , sodium , biochemistry , membrane transport , calcium , phosphorylation , membrane , biology , enzyme , organic chemistry , gene
The effect of exogenous adenosine triphosphate (ATP) and other nucleotides on the transport of Na in various mammalian red cells has been studied. While they have no effect on the transport of Na in human and cat red cells, in dog red cells adenosine and its mono‐, di‐, and triphosphorylated forms were found to increase Na‐influx. Of these, ATP has the most striking effect, causing a more than 8‐fold increase at a concentration of 0.6 mM and exerting this effect at a dose range of 10 −5 to 10 −3 M. The effect of ATP is rapid (>5 minutes) and can be reversed by washing or the addition of calcium or magnesium. In contrast to the adenosine series other phosphorylated nucleotides (GTP, CTP, UDP, GDP and cAMP) have no effect. The well known volume dependent Na‐transport in these cells is reversed in the presence of 0.6 mM ATP. It is suggested that ATP acts on passive cation movements either by chelation of membrane charge of by a direct interaction with membrane proteins and may be involved in the volume regulation of cation transport in the dog erythrocyte.