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Degradation of abnormal proteins in heLa cells
Author(s) -
Hendil Klavs B.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870304
Subject(s) - puromycin , cycloheximide , hela , incubation , protein biosynthesis , amino acid , biochemistry , degradation (telecommunications) , protein degradation , chemistry , biology , microbiology and biotechnology , cell , telecommunications , computer science
The experiments show that abnormal proteins are degraded faster than normal ones in HeLa cells. Among the fragmentary proteins made in the presence of puromycin, those with low molecular weight are least stable. Proteins made after incubation with 5‐fluorouracil or in the presence of some amino acid analogues are also unstable. Breakdown of proteins made in the presence or absence of puromycin is nearly unaffected by cycloheximide and is independent of pH between 7 and 8.