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Non‐histone protein synthesis during G 1 phase and its relation to dna replication
Author(s) -
Gerner Eugene W.,
Meyn Raymond E.,
Humphrey Ronald M.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870303
Subject(s) - chinese hamster ovary cell , dna synthesis , histone , kinetics , chromatin , dna replication , microbiology and biotechnology , biology , dna , plateau (mathematics) , chemistry , cell culture , biochemistry , genetics , physics , quantum mechanics , mathematical analysis , mathematics
The kinetics of non‐histone chromosomal protein (NHCP) synthesis were studied in Chinese hamster ovary (CHO) plateau phase cells stimulated to proliferate and were compared to NHCP synthesis kinetics in two populations of synchronous G 1 traversing cells. In all cases, NHCP synthesis rates increase 3‐ to 5‐fold as cells traversed G 1 and attained maximum values one hour before semi‐conservative DNA replication began. Similar to results in synchronous G 1 cells, the molecular weight distributions of the NHCP fraction from stimulated plateau phase cells underwent only minor changes, measured by sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis, as these cells moved toward S phase. Yet, during this progression after plateau phase and in the transition from early G 1 to late G 1 in synchronous cells, the total NHCP fraction increased significantly (1.5–2‐fold) in amount per cell. These data indicate that plateau phase cells are similar to early G 1 cells, both in terms of their amounts of non‐histone per cell and in their subsequent NHCP synthesis kinetics as they move toward S phase. These results extend previous findings which suggested that NHCP synthesis was coupled to DNA replication and demonstrate that the increased NHCP synthesis and accumulation in chromatin may be a biochemical marker for G 1 progression.