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Enhancement of phenotypic expression in cultured malignant liver epithelial cells by a complex medium
Author(s) -
Martyn Gunn J.,
Shinozuka Hisashi,
Williams Gary M.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870111
Subject(s) - phosphoenolpyruvate carboxykinase , bone canaliculus , phenotype , biology , in vitro , chemically defined medium , cell culture , microbiology and biotechnology , enzyme , parenchyma , biochemistry , chemistry , anatomy , botany , gene , genetics
The ability of two media identically supplemented with serum to maintain functional and structural differentiated properties of cultured malignant liver epithelial cells was compared. Reuber H35 hepatoma cells grown in a complex medium, Medium E, possessed the gluconeogenic enzyme phosphoenolpyruvate carboxykinase and bile canaliculi. When grown in Eagle's Minimal Essential Medium, the activity of phosphoenolpyruvate carboxykinase was reduced and bile canaliculi were not formed. This decrease in properties was restored when cells maintained in Minimal Essential Medium were returned to Medium E. It was concluded that the enhanced phenotypic expression was probably due to enrichment of the nutrient environment.