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The effect of Lanthanum on electrophoretic mobility and passive cation movements of the ehrlich ascites tumor cell
Author(s) -
Smith Thomas C.
Publication year - 1976
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040870107
Subject(s) - chemistry , ouabain , biophysics , surface charge , lanthanum , electrophoresis , tumor cells , incubation , sodium , biochemistry , biology , inorganic chemistry , organic chemistry , cancer research
We have investigated the effects of La +3 binding to the surface of Ehrlich ascites tumor cells on cell electrophoretic mobility and passive movements of Na + and K + . Incubation of tumor cells in La +3 ‐containing media results in a La +3 concentration‐dependent decrease in net surface charge negativity. At [La +3 ] greater than 0.5 mM, the net surface charge becomes positive with maximum positivity occurring at [La +3 ] = 0.9 mM. The effects of La +3 binding on passive Na + and K + movements were investigated by following 22 Na and K + losses from ouabain‐inhibited cells. Neither low (0.02) nor high (1.0 mM) [La +3 ] had any effect on the K + efflux rate coefficient. 22 Na losses from control and La +3 ‐treated cells were consistent with washouts from two cellular compartments. Low [La +3 ] (0.02 mM) was without effect on Na + loss from the cells. However, higher [La +3 ] (1.0 mM) resulted in a 48% inhibition of Na + loss from the more slowly exchanging compartment. These results are not consistent with simple electrostatic interactions exerting a major influence on the passive movements of Na + and K + . It is suggested that La +3 interacts with sites specific for Na + , perhaps involved in a carrier‐mediated exchange system.