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Modulation in the rates of incorporation of lipid precursors during the cell cycle
Author(s) -
Lingwood C. A.,
Thomas D. B.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860508
Subject(s) - mastocytoma , phospholipid , choline , fucose , biochemistry , chemistry , membrane , permeability (electromagnetism) , thymidine , biophysics , biology , galactose , dna , in vitro
The rates of incorporation of 3 H‐choline into phospholipid and of 3 H‐fucose into glycolipid have been measured during the cell cycle of the murine mastocytoma, P815Y, synchronized by either velocity sedimentation or excess thymidine blockade. The rate of 3 H‐choline incorporation into acid‐insoluble material exhibited two distinct maxima coincident with the early S and G 2 periods, whereas the rate of incorporation of 3 H‐fucose into lipid extractable material was maximal during the G 2 period. Variation in rate of incorporation of 3 H‐choline could not be accounted for by changes in membrane permeability.