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Incorporation of thymidine and iodeoxyuridine in mammalian cells in vitro
Author(s) -
Myers D. K.,
Feinendegan L. E.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860507
Subject(s) - thymidine , in vitro , uridine , dna , dna synthesis , chemistry , biochemistry , microbiology and biotechnology , biology , cell culture , tissue culture , rna , genetics , gene
The incorporation of labelled thymidine (dT) and iododeoxy‐uridine (IdU) into DNA was studied with tissue culture cells and with normal mouse cells in vitro. The rates of incorporation and the ratio dT/IdU incorporation both varied from one type of cell to another and from one suspending medium to another. Despite the known complexity of the regulation of DNA synthesis, the data for incorporation of exogenous dT and IdU could be fitted reasonably well by a model for single‐step enzymic process. Deviations from the theoretical predictions were minimal in the presence of fluorodeoxyuridine.