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Infrequent chromosome aberrations in syrian hamster cells following partial synchrony by amino‐acid deprivation
Author(s) -
Popescu N. C.,
Casto B. C.,
Dipaolo J. A.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860505
Subject(s) - chromatid , hamster , chinese hamster , biology , arginine , incubation , cell culture , microbiology and biotechnology , isoleucine , chromosome , dna synthesis , cell division , embryo , cell , genetics , amino acid , dna , biochemistry , leucine , gene
Incubation of Syrian hamster embryo secondary cell cultures in isoleucine or arginine deficient medium inhibited cell multiplication; 4–7% of the cells were synthesizing DNA compared to approximately 40% for cells in complete medium. After the deficient medium was replaced with complete medium, cells resumed multiplication and within 12 hours 65–70% of the cells entered into the S phase. A second peak of labeled cells, approximately 55%, occurred 24 hours after addition of complete medium. Chromosomal damage characteristic for cell lines grown in amino acid deficient medium did not occur with these Syrian hamster cells. Isoleucine or arginine deprivation resulted in chromosome or chromatid aberrations in only 2–5% of the cells, whereas chromosomes of control cells in complete medium occasionally exhibit chromatid gaps (> 1% of the cells).