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Anchorage dependent changes in transport of glucose, adenosine, uridine and leucine in 3T3 cells
Author(s) -
Otsuka Haruki,
Moskowitz Merwin
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860410
Subject(s) - uridine , leucine , adenosine , glucose transporter , chemistry , biochemistry , 3t3 cells , microbiology and biotechnology , biology , endocrinology , rna , amino acid , gene , insulin , transfection
There is a low uptake of leucine in suspension cultures of 3T3 cells relative to the uptake in sparse monolayer cultures. The pattern of uptake of deoxyglucose is similar in suspension and monolayer cultures and changing the medium or adding serum stimulates uptake under both culture conditions. The uptake of uridine and adenosine is greater in suspension culture than in monolayer culture. Cells do not multiply in suspension culture but do multiply in monolayer culture and thus there is a correlation between uptake of leucine and conditions which stimulate cell multiplication, but no correlation of the uptake of deoxyglucose, uridine and adenosine with these conditions.