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The stimulation of 2‐deoxy‐D‐glucose transport in control and virus‐transformed cells by ethidium bromide
Author(s) -
Soslau Gerald,
Nass Margit M. K.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860211
Subject(s) - ethidium bromide , cytochalasin b , glucose transporter , stimulation , incubation , chemistry , cell , neuraminidase , virus , biochemistry , cytochalasin , microbiology and biotechnology , biology , biophysics , enzyme , virology , endocrinology , cytoskeleton , dna , insulin
Recently we demonstrated that ethidium bromide altered the plasma and subcellular membrane glycoproteins in control and virus transformed cells. It is reported here that ethidium bromide also stimulated the membrane associated process of sugar transport. The K m of the virus transformed cells and the ethidium bromide treated cells is the same as that of the control cells while the maximum velocity as compared to the control cells is significantly increased. The transport of 2‐deoxy‐D‐glucose was inhibited by glucose, cytochalasin B and neuraminidase but was unaffected by variations in cell density or pH of the incubation medium.