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Isolation and characterization of alkaline phosphatase‐constitutive variants from chinese hamster ovary cells (CHO‐K1)
Author(s) -
Nose Kiyoshi,
Katsuta Hajim
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860209
Subject(s) - chinese hamster ovary cell , alkaline phosphatase , biology , microbiology and biotechnology , hamster , enzyme , population , ovary , biochemistry , cell , cell culture , genetics , demography , sociology
Pure clones with high alkaline phosphatase (ALP) activity were isolated from Chinese hamster ovary (CHO‐K1) cells which showed no detectable ALP activity. ALP‐positive cells appeared at the frequency of 10 −4 in the N‐methyl‐N'‐nitro‐N‐nitrosoguanidine‐treated cell population. With respect for cellular morphology, plating efficiency and several other enzymatic activities, no distinct difference was found between the original CHO‐K1 cells and its ALP‐positive variants, although some alterations in karyotype were found. Levels of ALP activity in these clones was stably maintained during serial cultivation. Some of the enzymological properties of ALP in the isolated clones were similar to those in bone or kidney.