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Preparation and neutralization characteristics of an anti‐CSF antibody
Author(s) -
Shadduck Richard K.,
Metcalf Donald
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860208
Subject(s) - neutralization , antibody , chemistry , virology , biology , immunology
Abstract Nine of ten rabbits immunized with a partially purified L‐cell CSF had demonstrable titers of anti‐CSF activity. In vitro the antibody was markedly inhibitory to both post‐endotoxin mouse sera and several mouse tissue extracts. CSF containing conditioned media prepared from a number of sources showed variable inhibition suggesting that murine CSF's may be characterized by marked antigenic differences. Human sources of CSF were also inhibited thus indicating a degree of cross‐reactivity between murine and human factors. These studies may provide the initial steps toward definition of the role of CSF in vivo.