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Cell Attachment to cllagen: The requirement for energy
Author(s) -
Klebe Robert J.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860206
Subject(s) - divalent , cell , microbiology and biotechnology , chemistry , biophysics , cell culture , biochemistry , biology , genetics , organic chemistry
It has previously been demonstrated that cell attachment to collagen depends on the presence of a serum‐derived cell attachment factor. Two steps in the cell attachment process have been defined. First, the cell attachment factor can bind to collagen in the absence of divalent cations. Secondly, either Ca ++ or Mg ++ are required for cells to attach to the collagen‐cell attachment factor complex. A third requirement for cell attachment to collagen is demonstrated here; namely, cellular metabolic energy. A simple method for the assay of ATP in tissue culture cells is presented.