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ITP pyrophosphohydrolase and idp phosphohydrolase in rat tissue
Author(s) -
Vanderheiden Bernardo S.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860118
Subject(s) - enzyme , cytosol , biochemistry , kidney , intracellular , spleen , adenosine triphosphate , nucleoside , chemistry , biology , endocrinology , medicine , immunology
The existence of a nucleoside triphosphate pyrophosphohydrolase specific for ITP has been demonstrated in the cytosol fraction of a variety of rat tissues. The enzyme, stable to moderate heat treatment, was present in erythrocytes as well as brain, heart, kidney, liver, lung, muscle, ovaries, spleen, testes and thymus. The specific activity of the enzyme ranges from 26 to 150 m̈moles/min/g protein. In addition, evidence is given for a heat labile nucleoside diphosphate (IDP) phosphohydrolase present in most rat tissues, and particularly high in the adrenal (137 m̈moles/min/g protein). An “ITP‐IMP cycle” is proposed as a regulating mechanism for intracellular levels of ATP.