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Glutamine synthetase activity in WI‐38 cells
Author(s) -
Viceps Dace,
Cristofalo V. J.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040860103
Subject(s) - glutamine synthetase , glutamine , wi 38 , enzyme , biochemistry , hydrocortisone , enzyme assay , biology , growth medium , cell , cell growth , microbiology and biotechnology , ploidy , amino acid , bacteria , genetics , gene
Abstract In the presence of complete growth media (Eagle's MEM), human diploid WI‐38 cells have a low level of glutamine synthetase activity. The activity could be increased by depriving the cells of exogenous glutamine; addition of hydrocortisone to either glutamine‐deficient or complete medium had no effect on the activity of the enzyme. Cell growth ceased under conditions that enhanced glutamine synthetase activity, and hydrocortisone could not reverse this inhibition.