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The genetics and developmental regulation of L‐glycerol 3‐phosphate dehydrogenase
Author(s) -
Kozak Leslie P.,
Erdelsky Kathryn J.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850410
Subject(s) - locus (genetics) , dehydrogenase , allele , biology , genotype , microbiology and biotechnology , glycerol , biochemistry , phosphate , genetics , enzyme , gene
In addition to reviewing the genetic regulation of L‐glycerol 3‐phosphate dehydrogenase during development in the mouse, new evidence is presented that the electrophoretic properties of L‐glycerol 3‐phosphate dehydrogenase in Mus castaneus are determined by an allele ( d ) at the Gdc‐1 locus. Accordingly there are three alleles at the Gdc‐1 locus; the b allele in C57BL/6J mice differs from the d allele in electrophoretic properties and the c allele in BALB/cJ mice differs from the d allele with respect to both heat denaturation and electrophoretic properties. Identical segregation patterns of the L‐glycerol 3‐phosphate dehydrogenase phenotypes in liver, kidney, and skeletal muscle from offspring of an F 2 generation produced from parents with the c/d genotype suggest that the Gdc‐1 locus is the major structural locus for L‐glycerol 3‐phosphate dehydrogenase in these tissues. Heart muscle was pooled from mice of the F 2 generation with either c/c or d/d genotypes at the Gdc‐1 locus as determined by analysis of liver L‐glycerol 3‐phosphate dehydrogenase. The chromatographic properties of L‐glycerol 3‐phosphate dehydrogenase from the heart muscle was determined on DEAE‐cellulose ion exchange columns. The elusion profile of the L‐glycerol 3‐phosphate dehydrogenase indicates that the Gdc‐1 locus is also the major structural locus in heart muscle.