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Analysis of the induction and deinduction of tyrosine aminotransferase in enucleated HTC cells
Author(s) -
Ivarie R. D.,
Fan W. J.W.,
Tomkins G. M.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850404
Subject(s) - cycloheximide , tyrosine aminotransferase , enzyme , nucleus , intracellular , enzyme inducer , tyrosine , protein biosynthesis , cytoplasm , dexamethasone , hormone , messenger rna , tyrosine hydroxylase , biology , microbiology and biotechnology , chemistry , biochemistry , endocrinology , gene
Anucleate HTC cells have been used to determine the importance of the nucleus in the regulation of the intracellular levels of tyrosine aminotransferase (TAT) in hepatoma tissue culture (HTC) cells. In the absence of the nucleus, neither the induction of the enzyme by dexamethasone nor its deinduction upon removal of the hormone occurs. Degradation of the enzyme takes place when protein synthesis is inhibited in anucleates by cycloheximide. Therefore, the maintenance of induced levels of enzyme activity after dexamethasone withdrawal from pre‐induced anucleates suggests that the nucleus is required for the inactivation of the TAT mRNA.