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The pattern of density dependent growth inhibition in murine fibroblasts
Author(s) -
Canagaratna Mary C. P.,
Riley P. A.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850214
Subject(s) - term (time) , labelling , biology , dose dependence , microbiology and biotechnology , optical density , biophysics , chemistry , biochemistry , physics , endocrinology , optics , quantum mechanics
Observations on the pattern of nuclear incorporation of 3 H‐TdR in long term (8‐day) and short term (3‐day) 3T3 cultures with local cell densities between 0.2 × 10 4 and 6.2 × 10 4 cells/cm 2 are reported. Contrary to a number of previous studies our observations indicate that density dependent inhibition is exhibited in relatively sparse cultures, commencing at 0.5 × 10 4 cells/cm 2 . Various possible mechanisms which could have caused the observed pattern of density‐dependent regression in labelling index are discussed.