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The effect of insulin on basal and hormone‐induced elevations of cyclic AMP content in cultured human fibroblasts
Author(s) -
Rosenthal Judith W.,
Goldstein Samuel
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850210
Subject(s) - insulin , medicine , endocrinology , glucagon , intracellular , basal (medicine) , hormone , biology , chemistry , biochemistry
Abstract Studies were carried out on confluent cultures of human fibroblasts to explore the effect of insulin on basal and hormone‐induced elevations of intracellular cyclic AMP content during short‐term incubations in serum‐free medium. Insulin tended to decrease basal levels of cyclic AMP but this was not statistically significant. Similarly, insulin was unable to block the elevations of intracellular cyclic AMP content induced by PGE 1 , epinephrine and glucagon. Paradoxically, when cells were preincubated with insulin, PGE 1 ‐stimulated cyclic AMP elevation was potentiated, possibly because insulin was conserving factors needed for a maximal PGE 1 stimulus or retarding the leakage of cAMP itself. The results indicate that insulin has little or no direct effect on cyclic AMP metabolism in cultured human fibroblasts and is consistent with the known insensitivity of these cells to insulin for other parameters.