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The early effects of ouabain on potassium metabolism and rate of proliferation of mouse lymphoblasts
Author(s) -
Cuff Joyce M.,
Lichtman Marshall A.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850207
Subject(s) - ouabain , lymphoblast , potassium , chemistry , suspension culture , metabolism , staining , medicine , cell culture , endocrinology , biochemistry , biology , sodium , organic chemistry , genetics
Abstract Murine lymphoblasts grown in suspension culture in the presence of ouabain showed a dose dependent and sequential decrease in 86 Rb + (K + analogue) influx, cellular potassium content, and growth rate. An increase in eosin staining and a decrease in cell number was observed after two hours in the presence of 1 mM ouabain; 1 μM ouabain was without effect on any of the parameters measured. Ouabain inhibition was rapidly and completely reversible at concentrations that were not cytotoxic.