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Effect of leucine on the temperature sensitive phenotype of a mammalian leucyl‐tRNA synthetase mutant
Author(s) -
Molnar S. J.,
Rauth A. M.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850203
Subject(s) - chinese hamster ovary cell , leucine , mutant , amino acid , isoleucine , biochemistry , temperature sensitive mutant , biology , phenotype , chemistry , microbiology and biotechnology , gene , receptor
The concentration of leucine in the growth medium has been found to influence the expression of the temperature sensitive phenotype of a mutant of Chinese hamster ovary cells with an altered leucyl‐tRNA synthetase. Plating efficiency and growth studies showed that increasing the leucine concentration allows cells to survive at normally non‐permissive high temperatures and conversely decreasing the leucine concentration enhances the adverse effects of high temperature. A similar but smaller effect was noted with isoleucine. It is suggested that this observation may form the basis of a rapid test, useful in directing the investigation of the lesion in similar mutants to pathways involving specific amino acids.