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Phosphorylation but not transport of sugars is enhanced in virus‐transformed mouse 3T3 cells
Author(s) -
Colby C.,
Romano A. H.
Publication year - 1975
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040850103
Subject(s) - phosphorylation , 3t3 cells , intracellular , virus , glucose transporter , microbiology and biotechnology , transformation (genetics) , biology , kinase , biochemistry , chemistry , virology , transfection , gene , endocrinology , insulin
The transport and phosphorylation of 2‐deoxy‐D‐glucose are separate and sequential events in both normal and virus‐transformed 3T3 cells. The apparent enhancement of 2‐dOG uptake by 3T3 cells accompanying virus transformation is not due to an effect on the transport process but to enhanced phosphorylation by intracellular kinases. Phosphorylation of 3‐O‐methyl‐D‐glucose does not occur in these cells. Both the rate and extent of transport of this glucose analog is the same in normal cells, SV 40 virus‐transformed cells and sarcoma virus‐transformed cells. The appropriateness of using 3‐O‐MeG for studies of the glucose transport system of animal cells is examined and discussed.