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Agglutination by concanavalin A of normal chick embryo fibroblasts treated by 5‐bromodeoxyuridine (BrdU)
Author(s) -
Biquard JeanMichel
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840313
Subject(s) - rous sarcoma virus , bromodeoxyuridine , concanavalin a , embryo , biology , transformation (genetics) , thymidine , microbiology and biotechnology , agglutination (biology) , virus , virology , immunology , cell growth , antibody , biochemistry , dna , in vitro , gene
Abstract When chick embryo fibroblasts are grown for two days in the presence of low doses (18 μg/ml) of 5‐bromodeoxyuridine (BrdU), their agglu‐tinability by Concanavalin A is increased to about the same degree as following viral transformation of the cells by Rous sarcoma virus. Simultaneous addition of a large excess, but not of a low dose of thymidine prevents this effect of BrdU. Treatment with BrdU also enhances the agglutinability of fibroblasts infected with a conditional It mutant of Rcus sarcoma virus at the temperature of 41° which restricts morphological transformation.