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The membrane potentials of fibroblasts in different environments
Author(s) -
Bard J.,
Wright M. O.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840116
Subject(s) - membrane , membrane potential , embryonic stem cell , microbiology and biotechnology , human lung , biophysics , cell , fibroblast , cell membrane , chemistry , biology , cell culture , biochemistry , genetics , gene
The membrane potentials of human embryonic lung fibroblasts have been measured in different cellular environments. Sparse cells on plastic have a mean membrane potential of −8.5 mV. As the cells progress to dense culture, the mean membrane potential rises to −14.7 mV. The mean membrane potential of fibroblasts in human embryonic lung fragments by comparison was found to be −16.5 mV. Sparse cells on collagen, at the same density as the sparse cells on plastic, have mean membrane potentials of −10.8 mV. Sparse cells on plastic migrating from dense cellular areas, following a cut being made in a thick sheet of cells, have mean membrane potentials of −5.9 mV. The significance of these results in relation to cellular environments has been discussed.