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Calcium transport in isolated bone cells II. Calcium transport studies
Author(s) -
Dziak Rosemary,
Brand John S.
Publication year - 1974
Publication title -
journal of cellular physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.529
H-Index - 174
eISSN - 1097-4652
pISSN - 0021-9541
DOI - 10.1002/jcp.1040840110
Subject(s) - calcium , chemistry , extracellular , iodoacetamide , parathyroid hormone , biophysics , efflux , calcitonin , isotopes of calcium , oligomycin , calcium atpase , biochemistry , endocrinology , medicine , biology , atpase , enzyme , organic chemistry , cysteine
Calcium transport was studied in bone cells isolated from fetal rat calvaria. 45 Ca uptake experiments revealed an active component of calcium exchange. Calcium uptake was inhibited by iodoacetamide, DNP, CCCP and oligomycin and appeared to be dependent on medium phosphate concentration. Initial influx values exhibited saturation kinetics from 0.6 mM to 1.5 mM extracellular calcium. Efflux of 45 Ca from loaded cells increased in the presence of iodoacetamide, DNP and CCCP. Incubation of the cells af 4° C inhibited both influx and efflux of calcium. Parathyroid hormone had no consistent effect on calcium uptake although characteristic increases in cyclic AMP levels were seen with the hormone. Calcitonin appeared to cause a transient increase in calcium uptake.